Allosteric Inhibition of Glycolysis by Citrate

In the human biochemistry context, glycolysis is a critical metabolic pathway involved in the breakdown of glucose to produce energy. However, various factors can affect the efficiency and output of this pathway. Consider the following statements regarding inhibitory factors in glycolysis:

A researcher investigates how different compounds interact with enzymes in the glycolytic pathway and notices that one compound inhibits the enzyme phosphofructokinase (PFK-1). This enzyme is responsible for a key regulatory step in glycolysis. Additionally, the researcher observes that there are changes in the levels of certain metabolites that normally signal feedback inhibition.

Given this scenario, which of the following compounds is most likely to act as an allosteric inhibitor of PFK-1?