Effect of Substrate Concentration on Enzyme Activity

Medium Biochemistry Enzymes

Enzymes are biological catalysts that accelerate chemical reactions in the body. They typically work by lowering the activation energy required for a reaction to occur. Enzymes have specific active sites that bind substrates, and the specificity of this interaction is crucial for their function. Additionally, enzymes can be affected by various factors including temperature, pH, and the presence of inhibitors.

Consider a reaction catalyzed by enzyme X, where the substrate concentration is increased. If enzyme X follows Michaelis-Menten kinetics, which of the following statements is true regarding the effect of substrate concentration on the rate of reaction?

The rate of reaction will continuously increase without limit as substrate concentration increases.

The rate of reaction will increase until a maximum velocity (V_max) is reached.

The enzyme will become denatured as substrate concentration approaches saturation.

Substrate concentration has no effect on the rate of reaction.

Hint

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