Enzyme Activity and pH Levels: Experimental Design Analysis

A research study was conducted to explore the effects of different pH levels on enzyme activity. Researchers created three groups of enzyme solutions: one with a low pH of 3, one with a neutral pH of 7, and one with a high pH of 10. Each group was exposed to a substrate for a specific period to measure the rate of reaction. The researchers hypothesized that the enzyme activity would be highest at neutral pH and significantly lower at both acidic and basic pH levels.

During the experiment, multiple trials were conducted for each pH level to ensure reproducibility, and the results were averaged. The findings revealed that enzyme activity indeed peaked at a pH of 7, while significantly reduced activity was recorded at pH levels of 3 and 10. The researchers also noted some variability in results across trials.