A group of scientists is examining how varying concentrations of a sugar solution influence the rate of cellular respiration in yeast cells. In their experiment, they prepared five different solutions with concentrations of 0%, 5%, 10%, 15%, and 20%. The yeast was then added to each solution, and the scientists measured the rate of carbon dioxide production over a 30-minute period. They recorded the average rate of carbon dioxide produced as the main data point.
Based on this experimental design, which of the following statements best identifies a key element of the experimental setup?