A group of researchers investigated the effects of different types of dietary carbohydrates on weight gain in rats. They divided 40 rats into four groups of 10, each receiving a different carbohydrate diet for 6 weeks: Group A received simple sugars, Group B received starches, Group C received fiber-based carbohydrates, and Group D had no carbohydrates at all. The researchers measured the weight of each rat weekly and recorded their activity levels using a motion detector.
At the conclusion of the study, the researchers wanted to determine whether the type of carbohydrate consumed significantly affected the weight gain of the rats. They used a one-way ANOVA to analyze the data and found a significant difference between the groups. However, they did not report the specific statistical tests used for intergroup comparisons.
Based on this experimental design, what is a potential limitation of this study that could affect the validity of the conclusions drawn?