Role of F2,6BP in PFK-1 Regulation

Very Hard Biochemistry Carbohydrates

In mammalian biochemistry, carbohydrates serve various critical functions, including energy storage and structural roles. One of the critical enzymes responsible for regulating glucose metabolism is phosphofructokinase-1 (PFK-1). It catalyzes the conversion of fructose-6-phosphate (F6P) to fructose-1,6-bisphosphate (F1,6BP) in the glycolytic pathway.

This reaction is particularly important because PFK-1 is a key regulatory step in glycolysis and is allosterically regulated by several metabolites. Consider a scenario where fructose-2,6-bisphosphate (F2,6BP), an important allosteric activator of PFK-1, is present at high levels. Which of the following statements correctly describes the impact of F2,6BP on PFK-1 activity?

F2,6BP increases the phosphorylation of PFK-1, leading to decreased enzymatic activity.

F2,6BP acts as a competitive inhibitor of PFK-1, reducing its activity.

F2,6BP enhances the affinity of PFK-1 for its substrate, fructose-6-phosphate, increasing its activity.

F2,6BP decreases the levels of fructose-1,6-bisphosphate, thereby inhibiting PFK-1.

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