Role of Helicase in DNA Replication

Very Hard Biochemistry Nucleic Acids And Dna Replication

During DNA replication, several key enzymes are involved in ensuring the accurate and efficient duplication of the genetic material. One crucial step in this process is the unwinding of the double helix, which allows the replication machinery to access the template strands.

In bacteria, which protein is primarily responsible for initiating the unwinding of DNA at the replication origin, and what is the primary mechanism by which it functions?

DNA polymerase – It cleaves hydrogen bonds between base pairs.

Helicase – It utilizes ATP hydrolysis to break hydrogen bonds.

Topoisomerase – It cuts and rejoins the DNA to relieve torsional strain.

Single-stranded binding protein – It prevents re-annealing of the DNA.

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